Variations of sulfhydryl compounds of saliva and plasma in healthy population of different ages / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To analyze the effect of age and gender on sulfhydryl compounds content in saliva and plasma in healthy population and to study the relationship between sulfhydryl compounds content of saliva and plasma to provide a basis for clinical examination of saliva sulfhydryl compounds.</p><p><b>METHODS</b>Sulfhydryl compounds content of saliva and plasma were measured in 306 healthy adults from the Department of Clinical Laboratory of Health Management lnstitute of General Hospital of Chinese PLA (151 female and 155 male) who were divided into young group (20-44 years old, n = 106, 48 female and 58 male), middle-aged group (45-59 years old, n = 109, 63 female and 46 male) and elderly group (60-79 years old, n = 91, 40 female and 51 male).</p><p><b>RESULTS</b>Sulfhydryl compounds content in saliva and plasma in 306 healthy adults were (123±27) and (427±124) µmol/L respectively. Sulfhydryl compounds content in saliva and plasma were significantly decreased as age increased (both P < 0.01). Significant differences of sulfhydryl compounds content of saliva and plasma among the young group, middle-aged group and elderly group were found (P < 0.01). No sex difference was observed in saliva sulfhydryl compounds content (P = 0.451), however the sex difference was significant in plasma sulfhydryl compounds content (P = 0.006). There was a significantly positive correlation between sulfhydryl compounds content in saliva and plasma (r = 0.5050, P < 0.01).</p><p><b>CONCLUSIONS</b>Saliva sulfhydryl compounds content can roughly reflect plasma sulfhydryl compounds content. Saliva sulfhydryl compounds test is a promising biological index of aging which could be an alternative of plasma test.</p>

Effect of the peri-implantitis on the biological function of osteoblasts obtained from the mandibles / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effect of peri-implantitis inflammatory microenvironment on the biological function of jaw bone osteoblasts.</p><p><b>METHODS</b>Primary mandible osteoblasts from peri-implantitis and normal tissue were isolated and cultured. Third-generation purified osteoblasts were identified and detected. The proliferative activity of osteoblasts was evaluated through MTT assay. Osteocalcin (OCN), Runx2, and collagen I (Col I) mRNA levels were examined by real-time quantitative polymerase chain reaction. OCN protein levels were determined by Western blot.</p><p><b>RESULTS</b>: After 4 d of culture, the proliferative activity of osteoblasts from peri-implantitis became lower than that of normal tissue ( P <0.05). After 7 d of culture, OCN, Runx2, and Col I mRNA expression decreased ( P <0.05). The OCN protein levels also decreased ( P <0.05).</p><p><b>CONCLUSION</b>Peri-implantitis inflammatory microenvironment can decrease the proliferation and differentiation activity of mandible osteoblasts.</p>

Danggui-shaoyao-san, a traditional Chinese medicine prescription, alleviates the orthodontic pain and inhibits neuronal and microglia activation / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>The pain caused by orthodontic treatment has been considered as tough problems in orthodontic practice. Danggui-shaoyao-san (DSS) is a traditional Chinese medicine (TCM) prescription which has long been used for pain treatment and possesses antioxidative, cognitive enhancing and antidepressant effects. We raise the hypothesis that DSS exerts analgesic effect for orthodontic pain via inhibiting the activations of neuron and microglia.</p><p><b>METHODS</b>DSS was given twice a day from day 5 prior to experimental tooth movement (ETM). Directed face grooming and vacuous chewing movements (VCM) were evaluated. Immunofluorescent histochemistry and Western blot analysis were used to quantify the Iba-1 (microglia activation) and Fos (neuronal activation) expression levels in the trigeminal spinal nucleus caudalis (Vc).</p><p><b>RESULTS</b>ETM significantly increased directed face grooming and VCM which reached the peak at post-operative day (POD) 1 and gradually decreased to the baseline at POD 7. However, a drastic peak increase of Fos expression in Vc was observed at 4 hours and gradually decreased to baseline at POD 7; while the increased Iba-1 level reached the peak at POD 1 and gradually decreased to baseline at POD 7. Furthermore, pre-treatment with DSS significantly attenuated the ETM induced directed face grooming and VCM as well as the Fos and Iba-1 levels at POD 1.</p><p><b>CONCLUSION</b>Treatment with DSS had significant analgesic effects on ETM-induced pain, which was accompanied with inhibition of both neuronal and microglial activation.</p>

Determination of pazufloxacin concentration in saliva, gingival crevicular fluid and serum of healthy adults using high-performance liquid chromatography / 南方医科大学学报

<p><b>OBJECTIVE</b>To establish a rapid and sensitive high-performance liquid chromatography (HPLC) method for detecting pazufloxacin concentrations in the saliva, gingival crevicular fluid and serum of healthy adults.</p><p><b>METHODS</b>Samples of saliva, gingival crevicular fluid and serum were obtained from healthy adults receiving intravenous infusion of pazufloxacin. The concentrations of pazufloxacin in the samples were quantified by HPLC equipped with a reversed-phase column (Agilent Zorbax SB-C18 5 µm, 250 mm×4.6 mm). The mobile phase for pazufloxacin was a mixture of acetonitrile and 0.5% phosphoric acid containing 1% triethylamine (155:850), and 20 µl of the resulting solution was injected into the HPLC system at a flow rate of 1.0 ml/min. The detection wavelength was set at 245 nm. The samples were first deproteinized by precipitation with methanol followed by supernatant drying; the residue was reconstituted with the mobile phase and centrifuged, and the supernatants were directly injected into the HPLC system.</p><p><b>RESULTS</b>Pazufloxacin in the samples were totally separated without interference by any endogenous substances. The calibration curves showed a good linear regression (r>0.999). The detection limit was 10 ng/ml with within-day and between-day coefficients of variation performance all below 5% and recovery rates all above 91%.</p><p><b>CONCLUSION</b>HPLC is both sensitive and selective for quantification of pazufloxacin in saliva, gingival crevicular fluid and serum.</p>

Expression of glucose transporter-1 and insulin receptor alpha1 in osteoblast obtained from diabetic rats' mandibles / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To explore the expression of glucose transporter (GLUT)-1 and insulin receptor (IR) alpha1 in osteoblast obtained from diabetic rats' mandibles.</p><p><b>METHODS</b>The expression of GLUT-1 and IR alpha1 of diabetic and control groups were measured by reverse transcription polymerase chain reaction, Western blot and immunohistochemistry stain.</p><p><b>RESULTS</b>The mRNA expressions of GLUT-1 and IR alpha1 of diabetic group were significantly higher than control group (P<0.05). The protein expressions of GLUT-1 and IR alpha1 were similar to the mRNA expressions.</p><p><b>CONCLUSION</b>Osteoblasts obtained from diabetic rats' mandibles keep the adaptation changes to hyperglycemia and hypoinsulinemia, which may contribute to their dysfunction.</p>

Experimental Study of Vascular Tissue Engineering / 中国康复理论与实践

@#Objective To obtain the experimental data of vascular tissue engineering.MethodsThe vascular endothelial cells (VEC) and vascular smooth muscle cells (VSMCs) were acquired and cultured, and then seeded on vascular tissue engineering materials. The porous gelatin-chitosan scaffold with VSMCs was subcutaneously implanted, followed by the observation of the cell growth ten days later.ResultsThe two kinds of cells were successfully cultured and their morpholoical and immunohistochemical characteristics were consistent with vascular endothelial and VSMCs respectively. The VSMCs could grow extensively on the scaffold after the in vivo implantation. The scaffold were wrapped by the fibrous tissue ten days later after the in vitro implantation of VSMCs. The seed cells grew in the scaffold, and the vessel cavity seen in the center of the scaffold, was quite different from the normal vessel structure.ConclusionIt is feasible to implant the VSMCs with fibrin gels into the living body. The vessels reconstructed, though different from the normal structure, is similar to the embryo of the vessels.

Elementary study on construction of engineered blood vessel using chitosan-fibrin gel as scaffold / 中国康复理论与实践

@#ObjectiveTo investigate the feasibility of construction of engineered blood vessel using chitosan tube and fibrin gel as scaffold.MethodsVascular endothelial cells and smooth muscle cells were harvested from aortas of a rat, respectively. After expansion in vitro, vascular endothelial cells were seeded onto the inner surface of chitosan tube and smooth muscle cells mixed with fibrin gel seeded onto outer surface of the scaffold to construct engineered blood vessels. Inverted microscope, immunohistochemical staining and scanning electronic microscope were used to evaluate the construct.ResultsVascular endothelial cells formed monolayer and covered the inner surface of chitosan tube. Smooth muscle cells survived in the fibrin gel and grew in a 3-dimensional manner. ConclusionChitosan-fibrin gel may be potentially used as scaffold of engineered blood vessels.